Treatment for melanoma

ABSTRACT

The present invention relates to combination therapies for melanoma, and in particular, metastatic melanoma. Drugs for use in such therapies in include MEK inhibitors combination with cardiac glycosides.

The present application claims benefit of priority to U.S. Provisional Application Ser. No. 61/847,797, filed Jul. 18, 2013, the entire contents of which are hereby incorporated by reference.

BACKGROUND OF THE INVENTION

I. Technical Field

The present invention relates generally to the fields of medicine and oncology genetics. More particularly, it relates to the combined use of MEK inhibition and cardiac glycosides to treat melanoma, particularly metastatic melanoma.

II. Related Art

Melanoma is a malignant tumor of melanocytes. Melanocytes are cells that produce the dark pigment, melanin, which is responsible for the color of skin. They predominantly occur in skin, but are also found in other parts of the body, including the bowel, oral cavity and the eye. Melanin also protects the deeper layers of the skin from the sun's harmful ultraviolet (UV) rays. When people spend time in the sunlight, the melanocytes make more melanin and cause the skin to tan. This also happens when skin is exposed to other forms of ultraviolet light (such as in a tanning booth). If the skin receives too much ultraviolet light, the melanocytes suffer genetic damage and can be transformed into cancerous melanoma cells.

Because of the link to cumulative sun exposure, the chance of getting melanoma increases with age, but many young people also get melanoma. In fact, melanoma is one of the most common cancers in young adults. Each year in the U.S., more than 50,000 people—young and old—learn that they have melanoma. And, according to a WHO report, about 48,000 melanoma related deaths occur worldwide each year. The treatment includes surgical removal of the tumor, and if melanoma is found early while relatively small and thin, complete removal gives a high cure rate. The chance of the melanoma coming back or spreading depends on how deeply it has invaded into the layers of the skin. Thus, the more progressed the lesion, the great the chancer for recurrence and/or metastasis. For melanomas that recur or spread, treatments include chemo—plus immunotherapy or radiation therapy, but the prognosis for such patients, including those exhibiting metastatic disease (AJCC Stage III and IV) is poor, with 5-year survival rates being less than 10%. As such, improved treatments for melanoma, particularly for advanced metastatic disease, are urgently needed.

SUMMARY OF THE INVENTION

Thus, in accordance with the present invention, there is provided a method of inhibiting melanoma in a subject comprising administering to said subject (a) a MEK inhibitor; and (b) a cardiac glycoside, in an amount sufficient to inhibit said melanoma. The subject may be a human or a non-human mammal. Inhibiting may comprise inhibiting the growth of primary melanomas, inhibiting metastasis, inhibiting the growth of metastases, killing circulating melanoma cells, inducing remission, extending remission, or inhibiting recurrence. The melanoma may be AJCC stage III disease or AJCC stage IV disease. The subject may have previously received a radiotherapy, a chemotherapy, an immunotherapy, a molecularly targeted therapy or had surgical resection of a tumor. Administering may comprise topical, intravenous, intraarterial, subcutaneous, oral or intra-tumoral administration, or may comprise local, regional or systemic administration, or may comprise continuous infusion over a period of time. The subject may have failed one or more standard melanoma therapies.

The MEK inhibitor may be administered before the cardiac glycoside therapy, at the same time as the cardiac glycoside therapy or after the cardiac glycoside therapy. The MEK inhibitor may be administered more than once, and/or the cardiac glycoside may be administered more than once. The cardiac glycoside may be selected from digoxin, digitoxin, gitoxin, oleandrin, neriifolin, bufalin, marinobufagenin, cinobufagenin, UNBS1450 and lanatoside C. The MEK inhibitor may be selected from trametinib, AS703026 (Pimasertib, MSC1936369B), AZD8330 (ARRY-424704), Selumetinib (AZD6244), PD-325901, GDC-0973 (XL518) and CI-1040 (PD184352). The amount sufficient to inhibit said melanoma may be less than the amount required for inhibition by any single agent alone. The amount sufficient to inhibit said melanoma may be less toxic than the amount required for inhibition by any single agent alone.

The method may further comprising treating said subject with an additional agent, such as an immunomodulator (e.g., imiquimod, IL-2 or interferon), or a chemotherapeutic agent, such as dacarbazine. The method may also further comprise excising a melanoma lesion, where excising occurs prior to, after or concurrent with MEK inhibition/cardiac glycoside treatment. The meloma may be BRAF wild-type or BRAF mutated, such as where the BRAF mutation is V600E or V600K. The cardiac glycoside may be digitoxin or is digoxin. The immunomodulator may be an antibody against PD1 or CTLA4, such as Ipilimumab.

It is contemplated that any method or composition described herein can be implemented with respect to any other method or composition described herein.

The use of the word “a” or “an” when used in conjunction with the term “comprising” in the claims and/or the specification may mean “one,” but it is also consistent with the meaning of “one or more,” “at least one,” and “one or more than one.”

Other objects, features and advantages of the present invention will become apparent from the following detailed description. It should be understood, however, that the detailed description and the specific examples, while indicating specific embodiments of the invention, are given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.

BRIEF DESCRIPTION OF THE DRAWINGS

The following drawings form part of the present specification and are included to further demonstrate certain aspects of the present invention. The invention may be better understood by reference to one or more of these drawings in combination with the detailed description of specific embodiments presented herein.

FIGS. 1A-C. Therapeutically achievable and safe levels of digitoxin are sufficient for anti-melanoma activity. FIG. 1A, IC₅₀ values of digitoxin for various melanomas and human umbilical cord blood cells (hUCB). Melanomas that are sensitive to digitoxin at therapeutically achievable doses are indicated in orange. FIG. 1B, Representative dose response curves. FIG. 1C, Digitoxin concentrations measured in the plasma of the mice 4 hours after 0.5 mg/kg injection.

FIG. 2. Melanoma regression after treatment with a combination of MEK inhibitor (Trametinib, GSK1120212) and digitoxin. The effect of digitoxin alone, or in combination with the MEK inhibitor, was tested in xenograft assays using primary melanomas from patients. Melanoma cells were injected subcutaneously in the right flanks of immunocompromised NSG mice. When tumors became palpable, mice were randomized to four different treatments. Mice were treated either with digitoxin (0.5 mg/kg/day in 200 μl of 0.5% promethylcellulose, 0.2% Tween80 and 5% DMSO by oral gavage), or GSK1120212 (0.5 mg/kg/day in 200 μl of 0.5% promethylcellulose, 0.2% Tween80 and 5% DMSO by oral gavage) alone, or in combination. Control mice received vehicle only (200 μl 0.5% promethylcellulose, 0.2% Tween80 and 5% DMSO per day). Tumors were measured every 2-3 days with calipers.

FIGS. 3A-C. Digitoxin and MEK inhibitor combination is effective against melanoma from patient M481 (BRAF V600E). FIG. 3A, Representative bioluminescence images of M481 xenografts treated for 36 days with digitoxin and/or GSK1120212. FIG. 3B, the average tumor mass and standard deviation for each treatment. The statistical significance of differences between mean values was assessed by the Student's t-test. FIG. 3C, Melanomas excised and photographed at the end of treatments.

FIGS. 4A-C. Digitoxin and MEK inhibitor combination is effective against melanoma from patient M214 (BRAF wild-type). FIG. 4A, Representative bioluminescence images of M214 xenografts treated for 9 days with digitoxin and/or GSK1120212. FIG. 4B, The average tumor weight and standard deviation for each treatment. The statistical significance of differences between mean values was assessed by the Student's t-test. FIG. 4C, Melanomas excised and photographed at the end of treatments.

FIGS. 5A-D. Digoxin and MEK inhibitor combination is effective against M214 (BRAF wild-type). FIG. 5A, M214 melanoma cells were injected subcutaneously in the right flanks of immunocompromised NSG mice. When tumors became palpable, mice were randomized to four different treatments. Mice were treated either with MEKi (trametinib, 0.5 mg/kg/day in 200 μl of 0.5% promethylcellulose, 0.2% Tween80 by oral gavage) and/or digoxin (10 mg/kg/day with osmotic pump). Control mice received vehicle only (200 μl 0.5% promethylcellulose, 0.2% Tween80 by gavage and 50% DMSO with osmotic pump). Tumors were measured every 3-4 days with calipers. FIG. 5B, Average tumor weight (N=5) and standard deviation for each treatment. The statistical significance of differences between mean values was assessed by the Student's t-test. FIG. 5C, Melanomas excised and photographed at the end of treatment. FIG. 5D, Digoxin levels achieved in plasma of mice at the conclusion of therapy (28-days).

FIGS. 6A-D. Digoxin and MEK inhibitor combination is effective against M491 (BRAF V600E). FIG. 6A, M491 melanoma cells were injected subcutaneously in the right flanks of immunocompromised NSG mice. When tumors became palpable, mice were randomized to four different treatments. Mice were treated either with MEKi (trametinib, 0.5 mg/kg/day in 200 μl of 0.5% promethylcellulose, 0.2% Tween80 by oral gavage) and/or digoxin (10 mg/kg/day with osmotic pump). Control mice received vehicle only (200 μl 0.5% promethylcellulose, 0.2% Tween80 by gavage and 50% DMSO with osmotic pump). Tumors were measured every 3-4 days with calipers. FIG. 6B, Average tumor weight (N=5) and standard deviation for each treatment. The statistical significance of differences between mean values was assessed by the Student's t-test. FIG. 6C, Melanomas excised and photographed at the end of treatment. FIG. 6D, Digoxin levels achieved in plasma of mice at the conclusion of therapy (21-days).

FIGS. 7A-D. Digoxin and MEK inhibitor combination is effective against M481 (BRAF V600E). FIG. 7A, M481 melanoma cells were injected subcutaneously in the right flanks of immunocompromised NSG mice. When tumors became palpable, mice were randomized to four different treatments. Mice were treated either with MEKi (trametinib, 0.5 mg/kg/day in 200 μl of 0.5% promethylcellulose, 0.2% Tween80 by oral gavage) and/or digoxin (10 mg/kg/day with osmotic pump). Control mice received vehicle only (200 μl 0.5% promethylcellulose, 0.2% Tween80 by gavage and 50% DMSO with osmotic pump). Tumors were measured every 3-4 days with calipers. FIG. 7B, Average tumor weight (N=3-5) and standard deviation for each treatment. The statistical significance of differences between mean values was assessed by the Student's t-test. FIG. 7C, Melanomas excised and photographed at the end of treatments. FIG. 7D, Digoxin levels achieved in plasma of mice at the conclusion of therapy (15-days).

FIG. 8. Combination of digoxin and MEKi is differentially toxic to melanoma cells compared to normal cells. Melanomas (M498 and M214) and normal human cells (fibroblasts, melanocytes, cord blood cells, colonic epithelial cells and bronchial epithelial cells) were treated with 50 ng/mL digoxin and 25 nM trametinib for 72 h in quadriplicates. Viability was measured with CellTiter-Glo (Promega). Error bars denote standard deviation.

DETAILED DESCRIPTION OF THE INVENTION

In previous studies, the inventors have identified cardiac glycosides as highly potent anti-melanoma agents. Here, they extend this work in pre-clinical studies combining cardiac glycosides (e.g., digitoxin) with a MEK inhibitor (Trametinib) to inhibit human melanomas xenografted in mice. The data show that this combination of agents almost completely eradicated the tumors, whereas either agent alone slowed tumor growth, but did not cause net regression. The effect was observed regardless of the BRAF status of the tumors. Thus, the inventors propose this unique combination of agents as a new melanoma therapy. These and other aspects of the disclosure are described in greater detail below.

I. Cancer Therapeutic Agents

A. Cardiac Glycosides

Cardiac glycosides are drugs used in the treatment of congestive heart failure and cardiac arrhythmia. These glycosides are found as secondary metabolites in several plants, but also in some animals, such as the milkweed butterflies. Therapeutic uses of cardiac glycosides primarily involve the treatment of cardiac failure. Their utility results from an increased cardiac output by increasing the force of contraction. By increasing intracellular calcium as described below, cardiac glycosides increase calcium-induced calcium release and thus contraction.

Drugs such as ouabain and digoxin are cardiac glycosides. Digoxin from the foxglove plant is used clinically, whereas ouabain is used only experimentally due to its extremely high potency.

Normally, sodium-potassium pumps in the membrane of cells (in this case, cardiac myocytes) pump potassium ions in and sodium ions out. Cardiac glycosides inhibit this pump by stabilizing it in the E2-P transition state, so that sodium cannot be extruded: intracellular sodium concentration therefore increases. A second membrane ion exchanger, NCX, is responsible for ‘pumping’ calcium ions out of the cell and sodium ions in (3Na/Ca); raised intracellular sodium levels inhibit this pump, so calcium ions are not extruded and will also begin to build up inside the cell.

Increased cytoplasmic calcium concentrations cause increased calcium uptake into the sarcoplasmic reticulum via the SERCA2 transporter. Raised calcium stores in the SR allow for greater calcium release on stimulation, so the myocyte can achieve faster and more powerful contraction by cross-bridge cycling. The refractory period of the AV node is increased, so cardiac glycosides also function to regulate heart rate.

Binding of cardiac glycoside to Na—K ATPase is slow, and also, after binding, intracellular calcium increases gradually. Thus, the action of digitalis (even on IV injection) is delayed.

Raised extracellular potassium decreases binding of cardiac glycoside to Na—K ATPase. Consequently, increased toxicity of these drugs is observed in the presence of Hypokalemia.

1. Digoxin

Digoxin is a purified cardiac glycoside extracted from the foxglove plant, Digitalis lanata. Its corresponding aglycone is digoxigenin, and its acetyl derivative is acetyldigoxin. Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and sometimes heart failure that cannot be controlled by other medication. Digoxin preparations are commonly marketed under the trade names Lanoxin, Digitek, and Lanoxicaps. It is also available as a 0.05 mg/ml oral solution and 0.25 mg/ml or 0.5 mg/ml injectable solution. It is marketed by GlaxoSmithKline and many other pharmaceutical manufacturers.

Today, the most common indications for digoxin are atrial fibrillation and atrial flutter with rapid ventricular response. Beta blockers and/or calcium channel blockers should be the first choice. High ventricular rate leads to insufficient diastolic filling time. By slowing down the conduction in the AV node and increasing its refractory period, digoxin can reduce the ventricular rate. The arrhythmia itself is not affected, but the pumping function of the heart improves owing to improved filling.

The use of digoxin in heart problems during sinus rhythm was once standard, but is now controversial. In theory, the increased force of contraction should lead to improved pumping function of the heart, but its effect on prognosis is disputable, and other effective treatments are now available. Digoxin is no longer the first choice for congestive heart failure, but can still be useful in patients who remain symptomatic despite proper diuretic and ACE inhibitor treatment.

Digitalis/digoxin has recently fallen out of favor because it did not demonstrate a mortality benefit in patients with congestive heart failure; however, it did demonstrate a reduction in hospitalizations for this condition. Because other therapies have shown a mortality benefit in congestive heart failure, maximizing other therapies (e.g., beta blockers) first is recommended before using digoxin.

Digoxin is usually given by mouth, but can also be given by IV injection in urgent situations (the IV injection should be slow, and heart rhythm should be monitored). While IV therapy may be better tolerated (less nausea), digoxin has a very long distribution half-life into the cardiac tissue, which will delay its onset of action by a number of hours. The half-life is about 36 hours, and digoxin is given once daily, usually in 125-μg or 250-μg doses.

In patients with decreased kidney function, the half-life is considerably longer, calling for a reduction in dose or a switch to a different glycoside, such as digitoxin (not available in the United States), which has a much longer elimination half-life of around seven days, but is mainly eliminated from the body via the liver, and thus not affected by changes in kidney function.

Effective plasma levels vary depending on the medical indication. For congestive heart failure, levels between 0.5 and 1.0 ng/ml are recommended. This recommendation is based on post hoc analysis of prospective trials, suggesting higher levels may be associated with increased mortality rates. For heart rate control (atrial fibrillation), plasma levels are less defined and are generally titrated to a goal heart rate. Typically, digoxin levels are considered therapeutic for heart rate control between 1.0 and 2.0 ng/ml. In suspected toxicity or ineffectiveness, digoxin levels should be monitored. Plasma potassium levels also need to be closely controlled (see side effects below).

Quinidine, verapamil, and amiodarone increases plasma levels of digoxin (by displacing tissue binding sites and depressing renal digoxin clearance), so plasma digoxin must be monitored carefully.

The occurrence of adverse drug reactions is common, owing to its narrow therapeutic index (the margin between effectiveness and toxicity). Adverse effects are concentration-dependent, and are rare when plasma digoxin concentration is <0.8 μg/l. They are also more common in patients with low potassium levels (hypokalemia), since digoxin normally competes with K⁺ ions for the same binding site on the Na⁺/K⁺ ATPase pump.

Common adverse effects (≧1% of patients) include: loss of appetite, nausea, vomiting and diarrhea as gastrointestinal motility increases. Other common effects are blurred vision, visual disturbances (yellow-green halos and problems with color perception), confusion, drowsiness, dizziness, insomnia, nightmares, agitation, and depression, as well as a higher acute sense of sensual activities. Less frequent adverse effects (0.1%-1%) include: acute psychosis, delirium, amnesia, convulsions, shortened QRS complex, atrial or ventricular extrasystoles, paroxysmal atrial tachycardia with AV block, ventricular tachycardia or fibrillation, and heart block. Rarely, digoxin has been shown to cause thrombocytopenia. Gynaecomastia (enlargement of breast tissue) is mentioned in many textbooks as a side effect, thought to be due to the estrogen-like steroid moiety of the digoxin molecule, but when systematically sought, the evidence for this is equivocal. The pharmacological actions of digoxin usually result in non-toxic electrocardiogram changes, including ST depression or T wave inversion. PR interval prolongation, however, may be a sign of digoxin toxicity. Additionally, increased intracellular Ca²⁺ may cause a type of arrhythmia called bigeminy (coupled beats), eventually ventricular tachycardia or fibrillation. The combination of increased (atrial) arrhythmogenesis and inhibited atrioventricular conduction (for example paroxysmal atrial tachycardia) is said to be pathognomonic (i.e., diagnostic) of digoxin toxicity. An often described, but rarely seen, adverse effect of digoxin is a disturbance of color vision (mostly yellow and green) called xanthopsia. Digoxin has potentially dangerous interactions with verapamil, amiodarone, erythromycin, and epinephrine (as would be injected with a local anesthetic).

The main pharmacological effects of digoxin are on the heart. Extracardiac effects are responsible for some of the therapeutic and many of the adverse effects. It has mechanical effects as it increases myocardial contractility; however, the duration of the contractile response is just slightly increased. Overall, the heart rate is decreased, while blood pressure increases as the stroke volume is increased, leading to increased tissue perfusion. Myocardial efficiency is due to improved hemodynamics, and the ventricular function curve is improved.

Other, electrical effects are an initial brief increase in action potential, followed by a decrease as the K⁺ conductance increases due to an increased intracellular amounts of Ca²⁺ ions. The refractory period of the atria and ventricles is decreased, while it increases in the sinoatrial and AV nodes. A less negative resting membrane potential is made, leading to increased excitability. Other, more indirect effects are cholinomimetic because of vagal stimulation, giving rise to AV nodal delay.

The conduction velocity increases in the atria, but decreases in the AV node. The effect upon Purkinje fibers and ventricles is negligible. Automaticity is also increased, in the atria, AV node, Purkinje fibers and ventricles. ECG changes are increased PR interval, due to decreased AV conduction, and a decreased QT interval because of the altered duration of decreased action potential. Also, the T wave is inverted, accompanied by ST depression. It may cause AV junctional rhythm and ectopic beats (bigeminy) resulting in ventricular tachycardia and fibrillation. Slight vasodilation is seen in heart failure. This effect is contrary to effects that should be seen as a result of increased intracellular calcium levels, but this occurs since digoxin improves hemodynamics, which leads to restored angiotensin levels and decreased sympathetic discharge, causing indirect vasodilation. Digoxin also affects the kidney by increased renal blood flow and increased glomerular filtration rate. A mild diuretic effect is seen only in heart failure.

Digoxin binds to a site on the extracellular aspect of the α-subunit of the Na⁺/K⁺ ATPase pump in the membranes of heart cells (myocytes) and decreases its function. This causes an increase in the level of sodium ions in the myocytes, which leads to a rise in the level of intracellular calcium ions. This occurs because the sodium/calcium exchanger on the plasma membrane depends on a constant inward sodium gradient to pump out calcium. Digoxin decreases sodium concentration gradient and the subsequent calcium outflow, thus raising the calcium concentration in myocardiocytes and pacemaker cells.

Increased intracellular calcium lengthens phase 4 and phase 0 of the cardiac action potential, which leads to a decrease in heart rate. Increased amounts of Ca²⁺ also leads to increased storage of calcium in the sarcoplasmic reticulum, causing a corresponding increase in the release of calcium during each action potential. This leads to increased contractility, the force of contraction, of the heart.

There is also evidence that digoxin increases vagal activity, thereby decreasing heart rate by slowing depolarization of pacemaker cells in the AV node. This negative chronotropic effect would therefore be synergistic with the direct effect on cardiac pacemaker cells. Digoxin is used widely in the treatment of various arrhythmias.

2. Digitoxin

Digitoxin has similar structure and effects to digoxin (though the effects are longer-lasting). Unlike digoxin (which is eliminated from the body via the kidneys), it is eliminated via the liver, so could be used in patients with poor or erratic kidney function. However, it is now rarely used in current Western medical practice. While several controlled trials have shown digoxin to be effective in a proportion of patients treated for heart failure, the evidence base for digitoxin is not as strong, although it is presumed to be similarly effective. Digitoxin exhibits similar toxic effects to the more commonly used digoxin, namely: anorexia, nausea, vomiting, diarrhea, confusion, visual disturbances, and cardiac arrhythmias. Antidigoxin antibody fragments, the specific treatment for digoxin poisoning, are also effective in serious digitoxin toxicity. The first structural analysis of digitoxin was performed in 1925, but the full structure with an exact determination of the sugar groups was not accomplished until 1962.

3. Other Cardiac Glycosides

Gitoxin (C₄₁H₆₄O₁₄) is a secondary glycoside derived from Digitalis purpurea used in medicine for coronary disease. Other cardiac glycosides include oleandrin, neriifolin, bufalin, marinobufagenin, cinobufagenin, UNBS1450 and lanatoside C.

B. MEK Inhibitors

MEK is a dual-specificity kinase that phosphorylates the tyrosine and threonine residues on ERKs 1 and 2 required for activation. Two related genes encode MEK1 and MEK2 which differ in their binding to ERKs and, possibly, in their activation profiles. MEKs do not phosphorylate either SAPK or p38 MAPK. MEKs are substrates for several protein kinases including the Rafs (c-, A- and B-), Mos, Tpl-2, and MEKK1.

MEKs are phosphorylated by these kinases at two serine residues (218 and 222 in rat MEK1). Introduction of acidic residues and truncation of an alpha-helical region in the N-terminal domain causes constitutive activation of MEK. Such proteins are transforming. Mutation of the same residues to alanine generates dominant-negative proteins that have been used to determine the requirement for the ERK pathway in various processes. For example, when expressed in T cells of transgenic mice, T cell maturation and selection are disrupted.

MEK inhibitors inhibit MEK1 and/or MEK2. They can be used to inhibit the MAPK/ERK pathway which is often overactive in some cancers, such as melanoma. Hence MEK inhibitors have potential for treatment of some cancers, especially BRAF-mutated melanoma, and KRAS/BRAF mutated colorectal cancer. One MEK inhibitor is Trametinib (GSK1120212), which is FDA-approved to treat BRAF-mutated melanoma. It is also studied in combination with BRAF inhibitor dabrafenib to treat BRAF-mutated melanoma.

The Phase II Trametinib trial (NCT01245062) was conducted in patients with BRAF_(V600E/K) mutant advanced or malignant melanoma. Patients were randomized 2:1 to Trametinib (2 mg QD) or chemotherapy (dacarbazine or paclitaxel). Patients were stratified by baseline LDH level and prior chemotherapy; patients in the chemotherapy arm were allowed to crossover to receive Trametinib after confirmation of progressive disease. Primary endpoint was progression free survival in patients with BRAF_(V600E) mutation-positive malignant melanoma and no prior brain metastasis; secondary endpoints were overall survival, overall response rate and safety in primary and intention to treat groups. Progression free survival and overall survival were compared using a stratified log-rank test. The study was designed with ≧99% power and one-sided α=0.025 to detect 57% reduction in the risk of progressive disease or death in pts treated with Trametinib vs chemotherapy.

Between December of 2010 and July of 2011, 322 patients were randomized to Trametinib (n=214) or chemotherapy (n=108); 273 patients were BRAF_(V600E) mutation-positive with no prior brain metastasis. HR for primary population for progression free survival by investigator was 0.44 (95% CI 0.31-0.64; p<0.0001) in favor of Trametinib with a median progression free survival of 4.8 mo vs 1.4 mo with chemotherapy. Progression free survival benefit in favor of Trametinib was observed in the intention to treat group; this was confirmed by an independent review. The confirmed overall response rate was 24% with Trametinib and 7% with chemotherapy. HR for interim overall survival was 0.53 (95% CI 0.30-0.94; p=0.0181), in favor of Trametinib in primary population. Overall survival benefit was consistent in the intention to treat population despite 51 patients crossover from chemotherapy to Trametinib. The most frequent adverse events with Trametinib were skin rash, diarrhea, edema, hypertension, fatigue. Known MEKi class effects were observed, e.g., chorioretinopathy (<1%) and decreased ejection fraction (7%). Grade 3 adverse effects in the Trametinib arm were hypertenstion (12%) and rash (7%). The study concluded that Trametinib was the first in class MEKi associated with a significant improvement of progression free survival and overall survival compared to chemotherapy in pts with BRAF_(V600E/K) mutant malignant melanoma.

Others MEK inhibitors include selumetinib, which had a phase 2 clinical trial for non-small cell lung cancer (NSCLC), MEK162, which had a phase 1 trial for biliary tract cancer and melanoma, PD-325901, indicated for breast cancer, colon cancer, and melanoma, as well as XL518, CI-1040, AS703026 (Pimasertib, MSC1936369B), AZD8330(ARRY-424704), Selumetinib (AZD6244), and PD035901.

II. Methods of Treatment

In a particular aspect, the present invention provides methods for the treatment of melanoma. Treatment methods will involve administering to an individual having such a disease an effective amount of a composition containing the compounds of the present invention. An effective amount is described, generally, as that amount sufficient to detectably and repeatedly to ameliorate, reduce, minimize or limit the extent of the disease or its symptoms. More specifically, it is envisioned that the treatment with compounds of the present invention kill cancer cells, inhibit their growth, reduce or inhibit metastasis, inhibit or reduce or delay recurrence, or otherwise provide clinical benefit. Also, combinations my reduce toxicity due to lower dosing and or reduced frequency of administration.

A. Melanoma

Melanoma is less common than other skin cancers. However, it is much more dangerous if it is not found early. It causes the majority (75%) of deaths related to skin cancer. Worldwide, doctors diagnose about 160,000 new cases of melanoma yearly. It is more common in women than in men. In women, the most common site is the legs and melanomas in men are most common on the back. It is particularly common among Caucasians, especially northern Europeans living in sunny climates. There are high rates of incidence in Australia, New Zealand, North America (especially Texas and Florida), Latin America, and Northern Europe, with a paradoxical decrease in southern Italy and Sicily. This geographic pattern reflects the primary cause, ultraviolet light (UV) exposure crossed with the amount of skin pigmentation in the population.

1. Early Signs

Early signs of melanoma are changes to the shape or color of existing moles or, in the case of nodular melanoma, the appearance of a new lump anywhere on the skin (such lesions should be referred without delay to a dermatologist). At later stages, the mole may itch, ulcerate or bleed. Early signs of melanoma are summarized by the mnemonic “ABCDE”:

Asymmetry

Borders (irregular)

Color (variegated)

Diameter (greater than 6 mm (0.24 in), about the size of a pencil eraser)

Evolving over time

These classifications do not, however, apply to the most dangerous form of melanoma, nodular melanoma, which has its own classifications:

Elevated above the skin surface

Firm to the touch

Growing

Metastatic melanoma may cause nonspecific paraneoplastic symptoms, including loss of appetite, nausea, vomiting and fatigue. Metastasis of early melanoma is possible, but relatively rare: less than a fifth of melanomas diagnosed early become metastatic. Brain metastases are particularly common in patients with metastatic melanoma. It can also spread to the liver, bones, abdomen or distant lymph nodes.

2. Development

The earliest stage of melanoma starts when the melanocytes begin to grow out of control. Melanocytes are found between the outer layer of the skin (the epidermis) and the next layer (the dermis). This early stage of the disease is called the radial growth phase, and the tumor is less than 1 mm thick. Because the cancer cells have not yet reached the blood vessels lower down in the skin, it is very unlikely that this early-stage cancer will spread to other parts of the body. If the melanoma is detected at this stage, then it can usually be completely removed with surgery. When the tumor cells start to move in a different direction—vertically up into the epidermis and into the papillary dermis—the behavior of the cells changes dramatically.

The next step in the evolution is the invasive radial growth phase, which is a confusing term; however, it explains the next step in the process of the radial growth, when individual cells start to acquire invasive potential. This step is important—from this point on the melanoma is capable of spreading. The Breslow's depth of the lesion is usually less than 1 mm (0.04 in), the Clark level is usually 2.

The following step in the process is the invasive melanoma—the vertical growth phase (VGP). The tumor attains invasive potential, meaning it can grow into the surrounding tissue and can spread around the body through blood or lymph vessels. The tumor thickness is usually more than 1 mm (0.04 in), and the tumor involves the deeper parts of the dermis. The host elicits an immunological reaction against the tumor (during the VGP), which is judged by the presence and activity of the tumor infiltrating lymphocytes (TILs). These cells sometimes completely destroy the primary tumor; this is called regression, which is the latest stage of the melanoma development. In certain cases, the primary tumor is completely destroyed and only the metastatic tumor is discovered.

3. Detection

Visual diagnosis of melanomas is still the most common method employed by health professionals. Moles that are irregular in color or shape are often treated as candidates of melanoma. The diagnosis of melanoma requires experience, as early stages may look identical to harmless moles or not have any color at all. People with a personal or family history of skin cancer or of dysplastic nevus syndrome (multiple atypical moles) should see a dermatologist at least once a year to be sure they are not developing melanoma. There is no blood test for detecting melanomas. Metastatic melanomas can be detected by X-rays, CT scans, MRIs, PET and PET/CTs, ultrasound, LDH testing and photoacoustic detection.

Many melanomas present themselves as lesions smaller than 6 mm in diameter; and all melanomas were malignant on day 1 of growth, which is merely a dot. An astute physician will examine all abnormal moles, including ones less than 6 mm in diameter. Seborrheic keratosis may meet some or all of the ABCD criteria, and can lead to false alarms among laypeople and sometimes even physicians. An experienced doctor can generally distinguish seborrheic keratosis from melanoma upon examination, or with dermatoscopy.

Total body photography, which involves photographic documentation of as much body surface as possible, is often used during follow-up of high-risk patients. The technique has been reported to enable early detection and provides a cost-effective approach (being possible with the use of any digital camera), but its efficacy has been questioned due to its inability to detect macroscopic changes. The diagnosis method should be used in conjunction with (and not as a replacement for) dermoscopic imaging, with a combination of both methods appearing to give extremely high rates of detection.

Melanoma is divided into the following types:

-   -   Lentigo maligna     -   Lentigo maligna melanoma     -   Superficial spreading melanoma     -   Acral lentiginous melanoma     -   Mucosal melanoma     -   Nodular melanoma     -   Polypoid melanoma     -   Desmoplastic melanoma     -   Amelanotic melanoma     -   Soft-tissue melanoma     -   Melanoma with small nevus-like cells     -   Melanoma with features of a Spitz nevus     -   Uveal melanoma         Confirmation of the clinical diagnosis is achieved with a skin         biopsy. This is usually followed up with a wider excision of the         scar or tumor. Depending on the stage, a sentinel lymph node         biopsy is done, as well, although controversy exists around         trial evidence for this procedure. Treatment of advanced         malignant melanoma is performed from a multidisciplinary         approach.

4. Staging

Melanoma stages are listed below with their 5 year survival rates:

Stage 0: Melanoma in situ (Clark Level I), 99.9% survival

Stage I/II: Invasive melanoma, 85-99% survival

-   -   T1a: Less than 1.00 mm primary tumor thickness, without         ulceration, and mitosis <1/mm²     -   T1b: Less than 1.00 mm primary tumor thickness, with ulceration         or mitoses ≧1/mm²     -   T2a: 1.00-2.00 mm primary tumor thickness, without ulceration

Stage II: High risk melanoma, 40-85% survival

-   -   T2b: 1.00-2.00 mm primary tumor thickness, with ulceration     -   T3a: 2.00-4.00 mm primary tumor thickness, without ulceration     -   T3b: 2.00-4.00 mm primary tumor thickness, with ulceration     -   T4a: 4.00 mm or greater primary tumor thickness without         ulceration     -   T4b: 4.00 mm or greater primary tumor thickness with ulceration

Stage III: Regional metastasis, 25-60% survival

-   -   N1: Single positive lymph node     -   N2: Two to three positive lymph nodes or regional         skin/in-transit metastasis     -   N3: Four positive lymph nodes or one lymph node and regional         skin/in-transit metastases

Stage IV: Distant metastasis, 9-15% survival

-   -   M1a: Distant skin metastasis, normal LDH     -   M1b: Lung metastasis, normal LDH     -   M1c: Other distant metastasis or any distant metastasis with         elevated LDH

5. Prognosis

Features that affect prognosis are tumor thickness in millimeters (Breslow's depth), depth related to skin structures (Clark level), type of melanoma, presence of ulceration, presence of lymphatic/perineural invasion, presence of tumor-infiltrating lymphocytes (if present, prognosis is better), location of lesion, presence of satellite lesions, and presence of regional or distant metastasis. Certain types of melanoma have worse prognoses but this is explained by their thickness. Interestingly, less invasive melanomas even with lymph node metastases carry a better prognosis than deep melanomas without regional metastasis at time of staging. Local recurrences tend to behave similarly to a primary unless they are at the site of a wide local excision (as opposed to a staged excision or punch/shave excision) since these recurrences tend to indicate lymphatic invasion.

When melanomas have spread to the lymph nodes, one of the most important factors is the number of nodes with malignancy. Extent of malignancy within a node is also important; micrometastases in which malignancy is only microscopic have a more favorable prognosis than macrometastases. In some cases micrometastases may only be detected by special staining, and if malignancy is only detectable by a rarely employed test known as the polymerase chain reaction (PCR), the prognosis is better. Macrometastases in which malignancy is clinically apparent (in some cases cancer completely replaces a node) have a far worse prognosis, and if nodes are matted or if there is extracapsular extension, the prognosis is still worse.

When there is distant metastasis, the cancer is generally considered incurable. The five year survival rate is less than 10%. The median survival is 6 to 12 months. Treatment is palliative, focusing on life-extension and quality of life. In some cases, patients may live many months or even years with metastatic melanoma (depending on the aggressiveness of the treatment). Metastases to skin and lungs have a better prognosis. Metastases to brain, bone and liver are associated with a worse prognosis.

There is not enough definitive evidence to adequately stage, and thus give a prognosis for ocular melanoma and melanoma of soft parts, or mucosal melanoma (e.g., rectal melanoma), although these tend to metastasize more easily. Even though regression may increase survival, when a melanoma has regressed, it is impossible to know its original size and thus the original tumor is often worse than a pathology report might indicate.

6. Standard of Care Treatments

Excisional biopsies may remove the tumor, but further surgery is often necessary to reduce the risk of recurrence. Complete surgical excision with adequate surgical margins and assessment for the presence of detectable metastatic disease along with short- and long-term follow-up is standard. Often this is done by a wide local excision (WLE) with 1 to 2 cm margins. Melanoma-in-situ and lentigo malignas are treated with narrower surgical margins, usually 0.2 to 0.5 cm. Many surgeons consider 0.5 cm the standard of care for standard excision of melanoma-in-situ, but 0.2 cm margin might be acceptable for margin controlled surgery (Mohs surgery, or the double-bladed technique with margin control). The wide excision aims to reduce the rate of tumor recurrence at the site of the original lesion. This is a common pattern of treatment failure in melanoma. Considerable research has aimed to elucidate appropriate margins for excision with a general trend toward less aggressive treatment during the last decades.

Mohs surgery has been reported with cure rate as low as 77% and as high as 98% for melanoma-in-situ. CCPDMA and the “double scalpel” peripheral margin controlled surgery is equivalent to Mohs surgery in effectiveness on this “intra-epithelial” type of melanoma.

Melanomas that spread usually do so to the lymph nodes in the area of the tumor before spreading elsewhere. Attempts to improve survival by removing lymph nodes surgically (lymphadenectomy) were associated with many complications, but no overall survival benefit. Recently, the technique of sentinel lymph node biopsy has been developed to reduce the complications of lymph node surgery while allowing assessment of the involvement of nodes with tumor.

Although controversial and without prolonging survival, sentinel lymph node biopsy is often performed, especially for T1b/T2+ tumors, mucosal tumors, ocular melanoma and tumors of the limbs. A process called lymphoscintigraphy is performed in which a radioactive tracer is injected at the tumor site to localize the sentinel node(s). Further precision is provided using a blue tracer dye, and surgery is performed to biopsy the node(s). Routine hematoxylin and eosin (H&E) and immunoperoxidase staining will be adequate to rule out node involvement. Polymerase chain reaction (PCR) tests on nodes, usually performed to test for entry into clinical trials, now demonstrate that many patients with a negative sentinel lymph node actually had a small number of positive cells in their nodes. Alternatively, a fine-needle aspiration biopsy may be performed and is often used to test masses.

If a lymph node is positive, depending on the extent of lymph node spread, a radical lymph node dissection will often be performed. If the disease is completely resected, the patient will be considered for adjuvant therapy. Excisional skin biopsy is the management of choice. Here, the suspect lesion is totally removed with an adequate (but minimal, usually 1 or 2 mm) ellipse of surrounding skin and tissue. To avoid disruption of the local lymphatic drainage, the preferred surgical margin for the initial biopsy should be narrow (1 mm). The biopsy should include the epidermal, dermal, and subcutaneous layers of the skin. This enables the histopathologist to determine the thickness of the melanoma by microscopic examination. This is described by Breslow's thickness (measured in millimeters). However, for large lesions, such as suspected lentigo maligna, or for lesions in surgically difficult areas (face, toes, fingers, eyelids), a small punch biopsy in representative areas will give adequate information and will not disrupt the final staging or depth determination. In no circumstances should the initial biopsy include the final surgical margin (0.5 cm, 1.0 cm, or 2 cm), as a misdiagnosis can result in excessive scarring and morbidity from the procedure. A large initial excision will disrupt the local lymphatic drainage and can affect further lymphangiogram-directed lymph node dissection. A small punch biopsy can be used at any time where for logistical and personal reasons a patient refuses more invasive excisional biopsy. Small punch biopsies are minimally invasive and heal quickly, usually without noticeable scarring.

High-risk melanomas may require adjuvant treatment, although attitudes to this vary in different countries. In the United States, most patients in otherwise good health will begin up to a year of high-dose interferon treatment, which has severe side effects, but may improve the patient's prognosis slightly. However British Association of Dermatologist guidelines on melanoma state that interferon is not recommended as a standard adjuvant treatment for melanoma. A 2011 meta-analysis showed that interferon could lengthen the time before a melanoma comes back but increased survival by only 3% at 5 years. The unpleasant side effects also greatly decrease quality of life. In Europe, interferon is usually not used outside the scope of clinical trials.

Various chemotherapy agents also are used, including dacarbazine (also termed DTIC), immunotherapy (with interleukin-2 (IL-2) or interferon (IFN)), as well as local perfusion, are used by different centers. The overall success in metastatic melanoma is quite limited. IL-2 (Proleukin) is the first new therapy approved for the treatment of metastatic melanoma in 20 years. Studies have demonstrated that IL-2 offers the possibility of a complete and long-lasting remission in this disease, although only in a small percentage of patients. A number of new agents and novel approaches are under evaluation and show promise. Clinical trial participation should be considered the standard of care for metastatic melanoma.

For lentigo maligna treatment, standard excision is still being performed by most surgeons. Unfortunately, the recurrence rate is exceedingly high (up to 50%). This is due to the ill-defined visible surgical margin, and the facial location of the lesions (often forcing the surgeon to use a narrow surgical margin). The narrow surgical margin used, combined with the limitation of the standard “bread-loafing” technique of fixed tissue histology, result in a high “false negative” error rate, and frequent recurrences. Margin control (peripheral margins) is necessary to eliminate the false negative errors. If bread loafing is used, distances from sections should approach 0.1 mm to assure that the method approaches complete margin control.

Some melanocytic nevi, and melanoma-in-situ (lentigo maligna), have resolved with an experimental treatment: imiquimod (Aldara) topical cream, an immune enhancing agent. Some dermasurgeons are combining the 2 methods: surgically excising the cancer and then treating the area with Aldara cream postoperatively for three months.

Radiation therapy is often used after surgical resection for patients with locally or regionally advanced melanoma or for patients with unresectable distant metastases. It may reduce the rate of local recurrence but does not prolong survival. Radioimmunotherapy of metastatic melanoma is currently under investigation. Radiotherapy has a role in the palliation of metastatic melanoma.

B. Dosages

In certain embodiments, the compound or compounds of the present invention is/are administered to a subject. In another embodiment of the invention, the dose range of the compound(s) will be measured by body weight, for example, about 0.5 mg/kg body weight to about 500 mg/kg body weight. Those of skill will recognize the utility of a variety of dosage range, for example, 1 mg/kg body weight to 450 mg/kg body weight, 2 mg/kg body weight to 400 mg/kg body weighty, 3 mg/kg body weight to 350 mg/kg body weighty, 4 mg/kg body weight to 300 mg/kg body weight, 5 mg/kg body weight to 250 mg/kg body weighty, 6 mg/kg body weight to 200 mg/kg body weight, 7 mg/kg body weight to 150 mg/kg body weighty, 8 mg/kg body weight to 100 mg/kg body weight, or 9 mg/kg body weight to 50 mg/kg body weight. Further, those of skill will recognize that a variety of different dosage levels will be of use, for example, 1 mg/kg, 2 mg/kg, 3 mg/kg, 4 mg/kg, 5 mg/kg, 7.5 mg/kg, 10 mg/kg, 12.5 mg/kg, 15 mg/kg, 17.5 mg/kg, 20 mg/kg, 25 mg/kg, 30 mg/kg, 35 mg/kg, 40 mg/kg, 45 mg/kg, 50 mg/kg, 60 mg/kg, 70 mg/kg, 80 mg/kg, 90 mg/kg, 100 mg/kg, 120 mg/kg, 140 mg/kg, 150 mg/kg, 160 mg/kg, 180 mg/kg, 200 mg/kg, 225 mg/kg, 250 mg/kg, 275 mg/kg, 300 mg/kg, 325 mg/kg, 350 mg/kg, 375 mg/kg, 400 mg/kg, 450 mg/kg, 500 mg/kg, 550 mg/kg, 600 mg/kg, 700 mg/kg, 750 mg/kg, 800 mg/kg, 900 mg/kg, 1000 mg/kg, 1250 mg/kg, 1500 mg/kg, 1750 mg/kg, 2000 mg/kg, 2500 mg/kg, and/or 3000 mg/kg. Of course, all of these dosages are exemplary, and any dosage in-between these points is also expected to be of use in the invention. Any of the above dosage ranges or dosage levels may be employed for a compound or compounds of the present invention.

The treatments may include various “unit doses.” Unit dose is defined as containing a predetermined-quantity of the therapeutic composition calculated to produce the desired responses in association with its administration, i.e., the appropriate route and treatment regimen. The quantity to be administered, and the particular route and formulation, are within the skill of those in the clinical arts. Also of import is the subject to be treated, in particular, the state of the subject and the protection desired. A unit dose need not be administered as a single injection but may comprise continuous infusion over a set period of time.

As is well known in the art, a specific dose level of active compounds for any particular patient depends upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination, and the severity of the particular disease undergoing therapy. The person responsible for administration will determine the appropriate dose for the individual subject. Moreover, for human administration, preparations should meet sterility, pyrogenicity, general safety and purity standards as required by FDA Office of Biologics standards.

C. Formulations and Routes for Administration

Pharmaceutical compositions of the present invention comprise an effective amount of one or more candidate substance or additional agent dissolved or dispersed in a pharmaceutically acceptable carrier. The phrases “pharmaceutical or pharmacologically acceptable” refers to molecular entities and compositions that do not produce an adverse, allergic or other untoward reaction when administered to an animal, such as, for example, a human, as appropriate. The preparation of a pharmaceutical composition that contains at least one candidate substance or additional active ingredient will be known to those of skill in the art in light of the present disclosure, as exemplified by Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, incorporated herein by reference. Moreover, for animal (e.g., human) administration, it will be understood that preparations should meet sterility, pyrogenicity, general safety and purity standards as required by FDA Office of Biological Standards.

As used herein, “pharmaceutically acceptable carrier” includes any and all solvents, dispersion media, coatings, surfactants, antioxidants, preservatives (e.g., antibacterial agents, antifungal agents), isotonic agents, absorption delaying agents, salts, preservatives, drugs, drug stabilizers, gels, binders, excipients, disintegration agents, lubricants, sweetening agents, flavoring agents, dyes, such like materials and combinations thereof, as would be known to one of ordinary skill in the art (see, for example, Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, pp. 1289-1329, incorporated herein by reference). Except insofar as any conventional carrier is incompatible with the active ingredient, its use in the therapeutic or pharmaceutical compositions is contemplated.

The candidate substance may comprise different types of carriers depending on whether it is to be administered in solid, liquid or aerosol form, and whether it need to be sterile for such routes of administration as injection. The present invention can be administered intravenously, intradermally, intraarterially, intraperitoneally, intralesionally, intracranially, intraarticularly, intraprostaticaly, intrapleurally, intratracheally, intranasally, intravitreally, intravaginally, intrarectally, topically, intratumorally, intramuscularly, subcutaneously, subconjunctival, intravesicularlly, mucosally, intrapericardially, intraumbilically, intraocularally, orally, locally, via inhalation (e.g., aerosol inhalation), via injection, via infusion, via continuous infusion, via localized perfusion bathing target cells directly, via a catheter, via a lavage, in creams, in lipid compositions (e.g., liposomes), or by other method or any combination of the forgoing as would be known to one of ordinary skill in the art (see, for example, Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, incorporated herein by reference).

The actual dosage amount of a composition of the present invention administered to an animal patient can be determined by physical and physiological factors such as body weight, severity of condition, the type of disease being treated, previous or concurrent therapeutic interventions, idiopathy of the patient and on the route of administration. The practitioner responsible for administration will, in any event, determine the concentration of active ingredient(s) in a composition and appropriate dose(s) for the individual subject.

In any case, the composition may comprise various antioxidants to retard oxidation of one or more component. Additionally, the prevention of the action of microorganisms can be brought about by preservatives such as various antibacterial and antifungal agents, including but not limited to parabens (e.g., methylparabens, propylparabens), chlorobutanol, phenol, sorbic acid, thimerosal or combinations thereof.

The candidate substance may be formulated into a composition in a free base, neutral or salt form. Pharmaceutically acceptable salts, include the acid addition salts, e.g., those formed with the free amino groups of a proteinaceous composition, or which are formed with inorganic acids such as for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric or mandelic acid. Salts formed with the free carboxyl groups can also be derived from inorganic bases such as for example, sodium, potassium, ammonium, calcium or ferric hydroxides; or such organic bases as isopropylamine, trimethylamine, histidine or procaine.

In embodiments where the composition is in a liquid form, a carrier can be a solvent or dispersion medium comprising but not limited to, water, ethanol, polyol (e.g., glycerol, propylene glycol, liquid polyethylene glycol, etc.), lipids (e.g., triglycerides, vegetable oils, liposomes) and combinations thereof. The proper fluidity can be maintained, for example, by the use of a coating, such as lecithin; by the maintenance of the required particle size by dispersion in carriers such as, for example liquid polyol or lipids; by the use of surfactants such as, for example hydroxypropylcellulose; or combinations thereof such methods. In many cases, it will be preferable to include isotonic agents, such as, for example, sugars, sodium chloride or combinations thereof.

In other embodiments, one may use eye drops, nasal solutions or sprays, aerosols or inhalants in the present invention. Such compositions are generally designed to be compatible with the target tissue type. In a non-limiting example, nasal solutions are usually aqueous solutions designed to be administered to the nasal passages in drops or sprays. Nasal solutions are prepared so that they are similar in many respects to nasal secretions, so that normal ciliary action is maintained. Thus, in preferred embodiments the aqueous nasal solutions usually are isotonic or slightly buffered to maintain a pH of about 5.5 to about 6.5. In addition, antimicrobial preservatives, similar to those used in ophthalmic preparations, drugs, or appropriate drug stabilizers, if required, may be included in the formulation. For example, various commercial nasal preparations are known and include drugs such as antibiotics or antihistamines.

In certain embodiments the candidate substance is prepared for administration by such routes as oral ingestion. In these embodiments, the solid composition may comprise, for example, solutions, suspensions, emulsions, tablets, pills, capsules (e.g., hard or soft shelled gelatin capsules), sustained release formulations, buccal compositions, troches, elixirs, suspensions, syrups, wafers, or combinations thereof. Oral compositions may be incorporated directly with the food of the diet. Preferred carriers for oral administration comprise inert diluents, assimilable edible carriers or combinations thereof. In other aspects of the invention, the oral composition may be prepared as a syrup or elixir. A syrup or elixir, and may comprise, for example, at least one active agent, a sweetening agent, a preservative, a flavoring agent, a dye, a preservative, or combinations thereof.

In certain particular embodiments, an oral composition may comprise one or more binders, excipients, disintegration agents, lubricants, flavoring agents, and combinations thereof. In certain embodiments, a composition may comprise one or more of the following: a binder, such as, for example, gum tragacanth, acacia, cornstarch, gelatin or combinations thereof; an excipient, such as, for example, dicalcium phosphate, mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, magnesium carbonate or combinations thereof; a disintegrating agent, such as, for example, corn starch, potato starch, alginic acid or combinations thereof; a lubricant, such as, for example, magnesium stearate; a sweetening agent, such as, for example, sucrose, lactose, saccharin or combinations thereof; a flavoring agent, such as, for example peppermint, oil of wintergreen, cherry flavoring, orange flavoring, etc.; or combinations thereof the foregoing. When the dosage unit form is a capsule, it may contain, in addition to materials of the above type, carriers such as a liquid carrier. Various other materials may be present as coatings or to otherwise modify the physical form of the dosage unit. For instance, tablets, pills, or capsules may be coated with shellac, sugar or both.

Additional formulations which are suitable for other modes of administration include suppositories. Suppositories are solid dosage forms of various weights and shapes, usually medicated, for insertion into the rectum, vagina or urethra. After insertion, suppositories soften, melt or dissolve in the cavity fluids. In general, for suppositories, traditional carriers may include, for example, polyalkylene glycols, triglycerides or combinations thereof. In certain embodiments, suppositories may be formed from mixtures containing, for example, the active ingredient in the range of about 0.5% to about 10%, and preferably about 1% to about 2%.

Sterile injectable solutions are prepared by incorporating the active compounds in the required amount in the appropriate solvent with various other ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle which contains the basic dispersion medium and/or the other ingredients. In the case of sterile powders for the preparation of sterile injectable solutions, suspensions or emulsion, the preferred methods of preparation are vacuum-drying or freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered liquid medium thereof. The liquid medium should be suitably buffered if necessary and the liquid diluent first rendered isotonic prior to injection with sufficient saline or glucose. The preparation of highly concentrated compositions for direct injection is also contemplated, where the use of DMSO as solvent is envisioned to result in extremely rapid penetration, delivering high concentrations of the active agents to a small area.

The composition must be stable under the conditions of manufacture and storage, and preserved against the contaminating action of microorganisms, such as bacteria and fungi. It will be appreciated that endotoxin contamination should be kept minimally at a safe level, for example, less that 0.5 ng/mg protein.

In particular embodiments, prolonged absorption of an injectable composition can be brought about by the use in the compositions of agents delaying absorption, such as, for example, aluminum monostearate, gelatin or combinations thereof.

The skilled artisan is directed to “Remington's Pharmaceutical Sciences” 15th Edition, chapter 33, in particular pages 624-652. Some variation in dosage will necessarily occur depending on the condition of the subject being treated. The person responsible for administration will, in any event, determine the appropriate dose for the individual subject. Moreover, for human administration, preparations should meet sterility, pyrogenicity, general safety and purity standards as required by FDA Office of Biologics standards.

D. Treatment

In the context of the present invention, it is contemplated that the compounds may be used in combination with each other to more effectively treat melanoma. When multiple therapeutic agents are administered, as long as the dose of the additional therapeutic agent does not exceed previously quoted toxicity levels, the effective amounts of the additional therapeutic agent may simply be defined as that amount effective to exert a therapeutic effect when administered to an animal in combination with the primary agent. This may be easily determined by monitoring the animal or patient and measuring those physical and biochemical parameters of health and disease that are indicative of the success of a given treatment. Such methods are routine in animal testing and clinical practice.

To kill or slow the growth of a cancer cell using the methods and compositions of the present invention, one can provide to the subject the stated combination of agents. These compositions would be provided in a combined amount effective to achieve a therapeutic benefit (inhibition of cancer cell growth, reduction in tumor size, induction of apoptosis in a cancer cell, etc.). This process may involve administering a combination at the same time. This may be achieved by administering a single composition or pharmacological formulation that includes both agents, or by administering two distinct compositions or formulations, at the same time.

Alternatively, treatment with one agent may precede or follow the additional agent treatment by intervals ranging from minutes to weeks. In embodiments where the additional agent is administered separately to the patient, one would generally ensure that a significant period of time did not expire between the time of each delivery, such that the agents would still be able to exert an advantageously combined effect on the cell. In such instances, it is contemplated that one would contact the cell with both modalities within about 12-24 hr of each other and, more preferably, within about 6-12 hr of each other, with a delay time of only about 12 hr being most preferred. In some situations, it may be desirable to extend the time period for treatment significantly, however, where several days (2, 3, 4, 5, 6 or 7) to several weeks (1, 2, 3, 4, 5, 6, 7 or 8) lapse between the respective administrations.

It also is conceivable that more than one administration of either or both agents will be desired. Various combinations may be employed, where a MEK inhibitor is “A” and the cardiac glycoside is “B,” as exemplified below:

A/B/A B/A/B B/B/A A/A/B B/A/A A/B/B B/B/B/A B/B/A/B A/A/B/B A/B/A/B A/B/B/A B/B/A/A B/A/B/A B/A/A/B B/B/B/A A/A/A/B B/A/A/A A/B/A/A A/A/B/A A/B/B/B B/A/B/B B/B/A/B

E. Further Combinations

Additional combinations with the agents/combinations set out above also are contemplated. Agents or factors suitable for use in a further combined cancer therapy are any chemical compound or treatment method that induces DNA damage when applied to a cell. Such agents and factors include radiation and waves that induce DNA damage such as, γ-irradiation, X-rays, UV-irradiation, microwaves, electronic emissions, and the like. A variety of chemical compounds, also described as “chemotherapeutic agents,” function to induce DNA damage, all of which are intended to be of use in the combined treatment methods disclosed herein. Chemotherapeutic agents contemplated to be of use, include, e.g., adriamycin, 5-fluorouracil (5FU), etoposide (VP-16), camptothecin, actinomycin-D, mitomycin C, cisplatin (CDDP) and even hydrogen peroxide. The invention also encompasses the use of a combination of one or more DNA damaging agents, whether radiation-based or actual compounds, such as the use of X-rays with cisplatin or the use of cisplatin with etoposide.

In treating cancer according to the invention, one would contact a tumor or tumor cells with an agent according to the present invention along with the second agent or therapy. This may be achieved by irradiating the localized tumor site with radiation such as X-rays, UV-light, γ-rays or even microwaves. Alternatively, the tumor or tumor cells may be contacted with the agent by administering to the subject a therapeutically effective amount of a pharmaceutical composition comprising a compound such as, adriamycin, 5-fluorouracil, etoposide, camptothecin, actinomycin-D, mitomycin C, or more preferably, cisplatin. The agent may be prepared and used as a combined therapeutic composition, or kit, by combining it with a compound according to the present invention.

Agents that directly cross-link nucleic acids, specifically DNA, are envisaged to facilitate DNA damage leading to a synergistic, antineoplastic combination with compounds of the present invention. Agents such as cisplatin, and other DNA alkylating agents may be used. Cisplatin has been widely used to treat cancer, with efficacious doses used in clinical applications of 20 mg/m² for 5 days every three weeks for a total of three courses. Cisplatin is not absorbed orally and must therefore be delivered via injection intravenously, subcutaneously, intratumorally or intraperitoneally.

Agents that damage DNA also include compounds that interfere with DNA replication, mitosis and chromosomal segregation. Such chemotherapeutic compounds include adriamycin, also known as doxorubicin, etoposide, verapamil, podophyllotoxin, and the like. Widely used in a clinical setting for the treatment of neoplasms, these compounds are administered through bolus injections intravenously at doses ranging from 25-75 mg/m² at 21 day intervals for adriamycin, to 35-50 mg/m² for etoposide intravenously or double the intravenous dose orally.

Agents that disrupt the synthesis and fidelity of nucleic acid precursors and subunits also lead to DNA damage. As such a number of nucleic acid precursors have been developed. Particularly useful are agents that have undergone extensive testing and are readily available. As such, agents such as 5-fluorouracil (5-FU), are preferentially used by neoplastic tissue, making this agent particularly useful for targeting to neoplastic cells. Although quite toxic, 5-FU, is applicable in a wide range of carriers, including topical, however intravenous administration with doses ranging from 3 to 15 mg/kg/day being commonly used.

Particular agents contemplated for use in melanoma include interferon, IL-2, imiquimod, paclitaxel, vemurafenib, ipilimumab and dacarbazine.

III. Examples

The following examples are included to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

Example 1 Materials and Methods

Tissue Banking.

The tissue bank protocol used for this study was developed and approved jointly by the clinical director of the University of Michigan (UM) melanoma program, the UM Cancer Center director of tissue procurement, the UM chief of anatomic pathology, and UM director of the section of dermatopathology. The protocol was developed to avoid any compromise in patient care, pathologic diagnosis, tumor staging or treatment. Patient confidentiality was maintained by password and firewall protected access to all pertinent databases. Melanoma specimens were obtained with informed consent from all patients according to protocols approved by the Institutional Review Board of the UM Medical School (IRBMED approvals HUM00050754 and HUM00050085). All patients included in this study had clinically apparent melanoma disease (biopsy-proven stage II, III, or IV, or obvious clinical stage IV) from which a small (typically 2-5 mm) tissue sample not required for standard-of-care pathology assessment was obtained. Most of the melanomas in this study were regional stage III lymph node or skin/soft tissue disease with palpable, clinically enlarged node(s) or soft tissues, undergoing definitive surgical resection, with biopsy-proven (most often needle core) diagnosis confirmed before surgery. The tumors were then shared with the Morrison laboratory at the University of Texas Southwestern Medical Center under a material transfer agreement.

Tumor Cell Preparation.

Tumors were mechanically dissociated with a McIlwain tissue chopper (Mickle Laboratory Engineering) before sequential enzymatic digestion in 200 U/ml collagenase IV (Worthington) for 20 min followed by 0.05% trypsin-EGTA for 2 min, both at 37° C. DNase (50-100 U/mL) was added to reduce clumping of cells during digestion. Cells were filtered (40 μm cell strainer) to obtain a single cell suspension. Dead cells and debris were depleted by density centrifugation (1.1 g/ml Optiprep; Sigma) when necessary. Cells were always passaged in vivo (in immunocompromised mice as detailed below), not in vitro.

For analysis of circulating melanoma cells, blood was collected from each mouse by cardiac puncture, using a syringe pre-treated with citrate-dextrose solution (Sigma). Red blood cells were precipitated by Ficoll sedimentation following the manufacturer's instructions (Ficoll Paque Plus, GE Healthcare). Remaining cells were washed with HBSS (Invitrogen) prior to antibody staining and flow cytometric analysis.

All antibody staining was carried out for 20 min on ice, followed by washing and centrifugation. In order to select live human melanoma cells and to exclude endothelial and hematopoietic cells, tumors obtained directly from patients were stained with directly conjugated antibodies to human CD45 (HI30-APC; BD Biosciences), human CD31 (WM59-APC; eBiosciences), Glycophorin A (HIR2-APC; Biolegend) and HLA-A,B,C (G46-2.6-PE; BD Biosciences). Tumors or blood obtained from mice were stained with antibodies against mouse CD45 (30-F11-APC; eBiosciences), mouse CD31 (390-APC; Biolegend), mouse Ter119 (TER-119-APC; eBiosciences) and human HLA-A,B,C (G46-2.6-PE; BD Biosciences). Cells were resuspended in 10 μg/ml DAPI (Sigma) and sorted on a FACSAria (Becton Dickinson).

Transplanting Melanoma Cells.

After sorting, cells were counted and resuspended in staining medium (L15 medium containing 1 mg/ml BSA, 1% penicillin/streptomycin and 10 mM HEPES (pH7.4)) with 25% high protein Matrigel (product 354248; BD Biosciences). Subcutaneous injections of human melanoma cells were performed in each flank and the interscapular region of NOD.CB17 (NOD/SCID IL2Rγ^(null), NSG) mice (Jackson Laboratories) in a final volume of 50 μl. These experiments were performed according to protocols approved by the animal use committee at the University of Texas Southwestern Medical Center (protocol 2011-0118).

Lentiviral Transduction of Human Melanoma Cells.

Replication incompetent lentiviruses were generated by the cotransfection of 293T cells with three plasmids (fuw, vsv and delta), kindly provided by Dr. Alnawaz Rehemtulla (University of Michigan). Forty-eight hours after transfection, supernatant with virus was collected and passed through a 0.45 μm low binding filter. Virus was concentrated by ultracentrifugation and aliquots were frozen at −80° C. until use. Around 150,000 sorted human melanoma cells (obtained directly from the patients or from xenografts) were incubated for 4 h at 37° C., 6.5% CO₂ in a well of an ultra-low attachment 24-well plate (Corning) containing 300 μl of cell culture medium (50% DMEM-low glucose, 30% Neurobasal (Invitrogen), 15% chick embryo extract, 1% Penicillin/Streptomycin, 1% non-essential amino acids (Gibco), 117 nM retinoic acid, 50 μM 2-mercaptoethanol (Sigma), 1% N2 supplement, 2% B27 supplement (Gibco), 20 ng/mL recombinant human bFGF, 20 ng/mL IGF-1 (R&D Systems)). Twenty μl lentivirus and 10 μg/ml polybrene (Sigma) were added to the cultures. Cells were then washed twice with staining medium and approximately 25,000 cells (a mixture of infected and non-infected cells) were injected into NSG mice. After growing to 1-2 cm in diameter, tumors were excised, dissociated into a single cell suspension and luciferase-GFP+ cells were collected by flow cytometry for injections into secondary recipients. Metastasis was monitored by BLI in these secondary recipients.

Bioluminescence Imaging (BLI).

Mice were injected with 100 luciferase-GFP+ cells on the right flank and monitored until tumor diameters approached 20 mm, at which point they were imaged along with an uninjected control mouse on an IVIS Imaging System 200 Series (Calliper Life Sciences) using Living Image software. Mice were injected intraperitoneally with 100 μl of PBS containing 40 mg/ml D-luciferin monopotassium salt (Biosynth) ten minutes before imaging, followed by general anesthesia two minutes before imaging. Following imaging of the whole mouse, the mice were euthanized, and individual organs were quickly imaged. The exposure time of images ranged from 10 to 60 seconds. The bioluminescence signal was quantified using “region of interest” measurement tools in Living Image software. After imaging, tumors and organs were fixed in 10% neutral buffered formalin for histopathology.

Histopathology and Immunostaining.

Melanomas and mouse organs were fixed in 10% neutral buffered formalin, paraffin embedded, sectioned, and stained with hematoxylin and eosin for histopathology. Sections from paraffin-embedded tumors were stained for S100 and analysed by a dermatopathologist to confirm they were melanomas. Binding of anti-S100 antibody (DAKO) was carried out for 30 min at room temperature, detected by anti-rabbit secondary antibody (30 min at room temperature) and revealed using DAB chromagen after quenching endogenous peroxidases. Slides were counterstained with hematoxylin.

Example 2 Results

High throughput screening of chemical libraries on primary human melanoma cells obtained from surgical specimens revealed cardiac glycosides including digitoxin to inhibit growth of melanoma cells in vitro. Digitoxin, has a long history of use in the treatment of various heart conditions. Plasma concentrations of digitoxin between 13 nM and 45 nM need to be achieved for therapeutic benefit. The inventors used in vitro viability assays to determine whether doses that show activity against melanoma fall within this therapeutic window. In these assays, cells isolated from primary melanoma samples were treated with various doses of digitoxin or DMSO for 72 hours in triplicate. Viability was measured with CellTiter-Glo. These experiments revealed that therapeutically achievable doses of digitoxin have activity against 9 out of 16 melanomas (FIGS. 1A-B). In order to start testing the effect of digitoxin in vivo, the inventors determined conditions that would yield doses in the plasma of mice that fell within the therapeutic range in humans (FIG. 1C).

The effect of digitoxin alone, or in combination with the MEK inhibitor Trametinib (GSK1120212), on in vivo melanoma growth was tested in xenograft assays using primary melanomas from patients. In these experiments, metastatic melanoma cells were injected subcutaneously in the right flanks of immunocompromised NSG mice. When tumors became palpable, mice were randomized to four different treatments. Mice were treated either with digitoxin (0.5 mg/kg/day in 200 μl of 0.5% promethylcellulose, 0.2% Tween80 and 5% DMSO by oral gavage), or GSK1120212 (0.5 mg/kg/day in 200 μl of 0.5% promethylcellulose, 0.2% Tween80 and 5% DMSO by oral gavage) alone, or in combination. Control mice received vehicle only (200 μl 0.5% promethylcellulose, 0.2% Tween80 and 5% DMSO per day). Tumors were measured every 2-3 days with calipers and bioluminescence imaging was performed when the diameter of the tumors in vehicle treated mice reached 2 cm.

The mouse xenograft assays showed that digitoxin and GSK1120212 individually slowed the growth of human melanoma xenografts. However, the combination of digitoxin and GSK1120212 caused substantial regression in melanomas obtained from multiple patients, some with BRAF mutations and some without (FIG. 2). The effects were significantly better than digitoxin or GSK1120212 alone. No difference in efficacy for the combination was seen in BRAF mutant versus BRAF wild-type tumors. Representative results from melanoma M481 carrying the BRAF-V600E mutation demonstrates that the effectiveness of the combination therapy in decreasing the bioluminescence signal (FIG. 3A), final tumor weight (FIG. 3B) and size (FIG. 3C). Similar observations were made with a BRAF wild-type melanoma, M214 (FIGS. 4A-C).

Digoxin is another cardiac glycoside that is structurally similar to digitoxin and has been used for the treatment of various heart conditions. The digoxin and trametinib combination was more effective against melanomas from patient M214 (FIGS. 5A-D), M491 (FIGS. 6A-D) and M481 (FIGS. 7A-D) than trametinib alone. Importantly, the combination of digoxin and trametinib was more toxic to two primary human melanoma cells in culture than to a variety of normal human cell lines including fibroblasts, melanocytes, cord blood cells, colonic epithelial cells and bronchial epithelial cells (FIG. 8).

Although the present invention and its advantages have been described in detail, it should be understood that various changes, substitutions and alterations can be made herein without departing from the invention as defined by the appended claims. Moreover, the scope of the present application is not intended to be limited to the particular embodiments of the process, machine, manufacture, composition of matter, means, methods and steps described in the specification. As one will readily appreciate from the disclosure, processes, machines, manufacture, compositions of matter, means, methods, or steps, presently existing or later to be developed that perform substantially the same function or achieve substantially the same result as the corresponding embodiments described herein may be utilized. Accordingly, the appended claims are intended to include within their scope such processes, machines, manufacture, compositions of matter, means, methods, or steps. 

What is claimed:
 1. A method of inhibiting melanoma in a subject comprising administering to said subject: (a) trametinib; and (b) a cardiac glycoside selected from digoxin and digitoxin, in an amount sufficient to inhibit said melanoma.
 2. The method of claim 1, wherein said subject is a human.
 3. The method of claim 1, wherein said subject is a non-human mammal.
 4. The method of claim 1, wherein inhibiting comprises inhibiting the growth of primary melanomas, inhibiting metastasis, inhibiting the growth of metastases, killing circulating melanoma cells, inducing remission, extending remission, or inhibiting recurrence.
 5. The method of claim 1, wherein said melanoma is AJCC stage III or stage IV disease.
 6. The method of claim 1, wherein said subject has previously received a radiotherapy, a chemotherapy, an immunotherapy, a molecularly targeted therapy or had surgical resection of a tumor.
 7. The method of claim 1, wherein trametinib is administered before or after the cardiac glycoside therapy.
 8. The method of claim 1, wherein trametinib is administered at the same time as the cardiac glycoside therapy.
 9. The method of claim 1, wherein trametinib and/or cardiac glycoside is administered more than once.
 10. The method of claim 1, wherein said cardiac glycoside is digoxin.
 11. The method of claim 1, wherein administering comprises topical, intravenous, intraarterial, subcutaneous, oral or intra-tumoral administration.
 12. The method of claim 1, wherein administering comprises local, regional or systemic administration.
 13. The method of claim 1, wherein administering comprises continuous infusion over a period of time.
 14. The method of claim 1, wherein said subject has failed one or more standard melanoma therapies.
 15. The method of claim 1, wherein said cardiac glycoside is digitoxin.
 16. The method of claim 1, wherein said amount sufficient to inhibit said melanoma is (a) less than the amount required for inhibition by either single agent alone, or (b) less toxic than the amount required for inhibition by either single agent alone.
 17. The method of claim 1, further comprising treating said subject with an additional agent.
 18. The method of claim 17, wherein said additional agent is an immunomodulator.
 19. The method of claim 18, wherein said immunomodulator is an antibody against PD1 or CTLA4, imiquimod, IL-2 or interferon.
 20. The method of claim 17, wherein said additional agent is a chemotherapeutic agent.
 21. The method of claim 1, further comprising excising a melanoma lesion.
 22. The method of claim 21, wherein excising occurs prior to or after trametinib/cardiac glycoside treatment.
 23. The method of claim 21, wherein excising occurs concurrent with trametinib/cardiac glycoside treatment.
 24. The method of claim 1, wherein said melanoma is BRAF wild-type.
 25. The method of claim 1, wherein said melanoma is BRAF mutated.
 26. The method of claim 25, wherein the BRAF mutation is V600E or V600K. 